【目录】

 

综述 —— 3

　・ “何谓外泌体？” 金泽大学 纳米生命科学研究所 华山力成 教授············································································ 3

PS亲和法 —— 5

　・ 前言························································································································································································· 5

　・ 磷脂酰丝氨酸和Tim4新型外泌体提取法························································································································· 5

　・ 外泌体分离和纯化方法的比较··········································································································································· 5

　・ PS亲和法的性能数据··························································································································································· 6

　・ 【专栏】不能仅凭蛋白总量计算外泌体回收量的原因·································································································· 9

　・ 金泽大学 纳米生命科学研究所 华山教授问答集《关于PS亲和法的优点与常见问题》········································· 9

外泌体分离和纯化 —— 11
　・ MagCapture™ 外泌体提取试剂盒PS Ver.2 ·················································································································· 11
 

miRNA提取和纯化 —— 13
　・ 纯化细胞外囊泡（EV）用miRNA提取试剂盒················································································································· 13        

 

外泌体检测和定量 —— 14

　・ 使用PS亲和法开发ELISA试剂盒·········································································································································· 14

　・ 外泌体ELISA试剂盒选择表··················································································································································· 14

　・ PS Capture™ 外泌体ELISA试剂盒（抗小鼠IgG POD）······························································································· 15

　・ PS Capture™ 外泌体ELISA试剂盒（链霉亲和素HRP）································································································ 16

　・ CD9/63/81-Capture人外泌体ELISA试剂盒（链霉亲和素HRP）··············································································· 18

　・ PS Capture™ 外泌体流式试剂盒········································································································································ 20

　・ 抗外泌体标记物抗体（CD9/CD63/CD81）····················································································································· 22

外泌体的吸附抑制和冷冻保存剂 —— 24

　・ EV-Save™ 细胞外囊泡保存稳定剂······································································································································ 24

　・ EV-Save™ 细胞外囊泡保存稳定剂（动物体内实验用）································································································· 25

纯化外泌体—— 26
　・ COLO201细胞来源纯化物外泌体········································································································································ 26

 

培养基和培养器具——27
　・MSCulture™ High Growth基础培养基 / MSCulture™ High Growth添加剂··························································· 27

　・EV-Up™ 间充质干细胞外泌体生产用基础培养基，AF / EV-Up™ 间充质干细胞外泌体生产用添加剂，AF········ 28

　・UniWells™ 水平连接细胞共培养板······································································································································· 30

常见问题 —— 32

 

相关产品：
 

1. MagCapture™ 外泌体提取试剂盒

2. PS Capture™ 外泌体ELISA试剂盒（抗小鼠IgG POD）

3. PS Capture™ 外泌体流式试剂盒

4. MagCapture™ 系列用磁珠捕获磁力架

5.  外泌体相关抗体 CD63 单克隆抗体

6.  PS Capture™ 外泌体ELISA试剂盒（链霉亲和素HRP）

7.  EV-Save™ 细胞外囊泡吸附抑制剂

8.  细胞外囊泡标记，单克隆抗体

9.  抗CD63单克隆抗体（3-13），荧光标记

10.  UniWells™ 水平链接细胞共培养板